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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
LIS1-338, monoclonal
形狀
buffered aqueous solution
分子量
antigen 46 kDa
物種活性
zebrafish, chicken, rat, human, bovine, mouse
濃度
~2 mg/mL
技術
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a rat brain cytosol preparation
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
SMILES 字串
CCCCC\C=C\C\C=C\CCCCCCCC(=O)OCC(O)COP(O)(O)=O
InChI
1S/C21H39O7P/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-21(23)27-18-20(22)19-28-29(24,25)26/h6-7,9-10,20,22H,2-5,8,11-19H2,1H3,(H2,24,25,26)/b7-6+,10-9+
InChI 密鑰
ZQTAMPRZFOOEEP-AVQMFFATSA-N
基因資訊
human ... PAFAH1B1(5048)
mouse ... Pafah1b1(18472)
rat ... Pafah1b1(83572)
一般說明
Monoclonal Anti-LIS1 (mouse IgG1 isotype) is derived from the LIS1-338 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human LIS1 protein. The LIS1 protein (46 kDa) contains seven WD (tryptophan-aspartic acid) repeats, a motif shared by at least 140 known proteins.
The gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) encodes a protein named LIS1 (lissencephaly-1) that forms the α subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase (PAFAH). The gene is mapped to human chromosome 17p13.3.
特異性
By immunoblotting, the antibody prefers the phosphorylated form of the LIS1 molecule (46 kDa).
免疫原
recombinant human LIS1 protein.
應用
Monoclonal Anti-LIS1 antibody produced in mouse has been used in protein pulldown assay and immunofluorescence.
生化／生理作用
LIS1 is involved in cell regulation, including the β subunits of G-proteins (Gβ). This protein motif is likely to mediate protein-protein interactions. LIS1 is a protein that is highly conserved during evolution; bovine, mouse, and chicken proteins exceed 99% similarity.
The heterotrimeric enzyme, platelet-activating factor acteylhydrolase (PAFAH), catalyzes the cleavage of acetyl group at the SN-2 position of platelet-activating factor. The α subunit encoded by the gene PAFAH1B1 (platelet activating factor acetylhydrolase 1b regulatory subunit 1) interacts with tubulin and affects microtubule dynamics. Mutations in this gene have been linked to Miller–Dieker lissencephaly, a human brain malformation characterized by a smooth cerebral surface and a disordered organization of the cortical layers resulting from a defect in neuronal migration.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Armen J Moughamian et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(32), 13190-13203 (2013-08-09)
Long-range retrograde axonal transport in neurons is driven exclusively by the microtubule motor cytoplasmic dynein. The efficient initiation of dynein-mediated transport from the distal axon is critical for normal neuronal function, and neurodegenerative disease-associated mutations have been shown to specifically
Interaction between LIS1 and doublecortin, two lissencephaly gene products
Caspi M, et al.
Human Molecular Genetics, 9(15), 2205-2213 (2000)
Shusheng Wang et al.
The Journal of biological chemistry, 286(1), 587-593 (2010-11-09)
The nuclear distribution protein E (NudE) and nuclear distribution protein E-like (Nudel or Ndel1) interact with both lissencephaly 1 (Lis1) and dynein. These interactions are thought to be essential for dynein function. Previous studies have shown that the highly conserved
T Sapir et al.
European journal of biochemistry, 265(1), 181-188 (1999-09-22)
Lissencephaly, a severe brain malformation, may be caused by mutations in the LIS1 gene. LIS1 encodes a microtubule-associated protein (MAP) that is also part of the enzyme complex, platelet-activating factor acetylhydrolase. LIS1 is also found in a complex with two
Isolation of a Miller-Dieker lissencephaly gene containing G protein beta-subunit-like repeats.
Reiner O
Nature, 364, 717-721 (1993)
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