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一般說明
来自西非单叶豆(加纳籽)的凝集素具有A和B两种亚基,分别识别αGalNAc和αGal端基。凝集素从豆科灌木加纳籽(Griffonia simplicifolia)的种子中提取,是糖类结合蛋白。
應用
来自西非单叶豆(加纳籽)的凝集素已用于免疫组化和免疫荧光染色。
生化/生理作用
来自西非单叶豆(加纳籽)的凝集素可凝集人A和B血型细胞。它对αGalNAc-和αGal具有特异性,可作为试剂用于检测生物材料中的αGal表位。凝集素(来自西非单叶豆)可用作异种反应性抗原(αGal1−3Gal)和癌症的标志物。凝集素在受精、胚胎发生、炎症、癌细胞转移和宿主-寄生物识别中起重要作用。
BS-I对末端α-D-半乳糖残基具有主要亲和力,对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
其他說明
BS-I是一种四聚体凝集素,由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素:BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的,其对末端α-D-半乳糖残基具有独特的亲和力,而BSI-A4 具有血型A特异性,其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
外觀
含柠檬酸盐缓冲盐和氯化钙
分析報告
凝集活性以 μg/mL表示,并且使用磷酸盐缓冲盐水(pH6.8)对1mg/mL溶液进行连续稀释液来测定,对于每种凝集素,所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后,对2%合适的红细胞悬浮液进行凝集所需的最低浓度。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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