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生物源
bovine heart
種類
Type XVII
化驗
5.0—15.0 mg protein/mL (biuret)
形狀
buffered aqueous glycerol solution
比活性
≥400 units/mg protein
分子量
140 kDa
製造商／商標名
Sigma-Aldrich
技術
cell based assay: suitable
雜質
<10 μg/mg protein free ammonium
顏色
white
pH值
7.5
正離子痕跡
NH4+: ≤10 μg/mg protein
應用
life science and biopharma
異物活動
glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%
pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%
儲存溫度
2-8°C
基因資訊
cow ... LDHA(281274) , LDHB(281275)
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一般說明
Research area: Cell signaling. L-LacticDehydrogenase (LDH) is present in the cell cytoplasm. and is part of the glycolytic pathway. Lactate dehydrogenase (LDH) is a ubiquitous molecule found in plants, yeast, mammals, and microorganisms and is a member of the oxidoreductase family. It has five isozymes (LD1 to LD5) and this composition varies in different tissues. LD1 is present at higher concentrations in the heart, kidneys, and erythrocytes. LD5 is found in the liver and skeletal muscles.(2)
應用
来自牛心的L-乳酸脱氢酶已用于R2复合物Rvb1p-Rvb2p、 RecA 蛋白和肌质网Ca2+-ATPase (SERCA)的ATPase测定。
生化／生理作用
L-乳酸脱氢酶 (LDH) 同工型水平的变化是组织损伤诊断标记。 LDH水平可用于检测奶牛疾病、乳腺炎。基于来自牛心的LDH 的染料可支持分析应用。
还可催化其他 L-2-羟基单羧酸的氧化反应。
單位定義
在pH7.5，37℃条件下，一个单位每分钟可将1.0μmole的丙酮酸还原为 L-乳酸。
外觀
溶于含50％甘油的0.025M磷酸钾缓冲液（pH7.5）中
分析報告
双缩脲法蛋白测定
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
其他客户在看
Purified E255L mutant SERCA1a and purified PfATP6 are sensitive to SERCA-type inhibitors but insensitive to artemisinins
Cardi D, et al.
The Journal of Biological Chemistry, 285(34), 26406-26416 (2010)
Active displacement of RecA filaments by UvrD translocase activity
Petrova V, et al.
Nucleic Acids Research, 43(8), 4133-4149 (2015)
Ligand binding and protein dynamics in lactate dehydrogenase
Pineda JR, et al.
Biophysical Journal, 93(5), 1474-1483 (2007)
Simultaneous purification of L-malate dehydrogenase and L-lactate dehydrogenase from bovine heart by biomimetic-dye affinity chromatography
Labrou NE and Clonis YD
Bioprocess and Biosystems Engineering, 16(3), 157-161 (1997)
Determination of lactate dehydrogenase (LDH) activity in milk by a fluorometric assay
Larsen T
The Journal of Dairy Research, 72(2), 209-216 (2005)
实验方案
This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.
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