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生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
W1B10, monoclonal
形狀
buffered aqueous solution
分子量
antigen 100-140 kDa
包含
15 mM sodium azide
物種活性
chicken
技術
immunoprecipitation (IP): suitable
indirect immunofluorescence: suitable
western blot: 1:200 using denatured, non-reduced extract of cultured fibroblasts from chicken embryo lower leg
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
chicken ... ITGB1(374058)
一般說明
Monoclonal anti-β-1-Chicken Integrin (mouse IgG1 isotype) is derived from the W1B10 hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified chicken integrin from fibroblasts of 11 days old chicken embryos. Chicken integrin is a heterodimer consisting of the common β-1 subunit and two α subunits (α-5 or α-3). The β-1 subunit of chicken integrin is a 803-amino acid transmembrane glycoprotein consisting of a large extracellular domain and a short cytoplasmic domain.
特異性
The antibody recognizes an epitope on the extracellular side of the avian integrin complex, and inhibits adhesion of embryo and tendon fibroblasts as well as skeletal myoblasts to both laminin and fibronectin. Under reducing and denaturing conditions using extracts of chicken embryo muscle cells, the antibody precipitates the β1 class integrin heterodimer as a broad band of ~140 kDa. In the absence of reducing agents, the integrin β1 subunit appears as bands at 100 kDa (immature form), 100 kDa (mature form), and 140 kDa (noncovalently associated α-subunits). The antibody may also be used for bioactivity studies in adhesion assays.
免疫原
chicken integrin.1
應用
Anti-Integrin β1 antibody, Mouse monoclonal has been used in western blot, fluorescent activated cell sorter (FACS) analysis.
生化／生理作用
Integrin binding to extracellular matrix (ECM) ligands regulates cell adhesion, shape, motility, intracellular signalling and gene expression. The monoclonal anti-b-1 chicken integrin antibody (clone W1B10) has been used to inhibit chicken muscle cell adhesion in cell cultures.
外觀
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin with 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
nwg
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Madame Curie Bioscience Database
Magma (New York, N.Y.), 18(4), 343-355 (2013)
Bonnie M Marsick et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 32(1), 282-296 (2012-01-06)
The development of a functioning neural network relies on responses of axonal growth cones to molecular guidance cues that are encountered en route to their target tissue. Nerve growth factor (NGF) and neurotrophin-3 serve as attractive cues for chick embryo
L Cao et al.
Matrix biology : journal of the International Society for Matrix Biology, 18(4), 343-355 (1999-10-12)
We have observed that the spent culture media in suspended chondrocyte cultures is essential for the survival of the cells, since complete change of the spent media induces severe programmed cell death (apoptosis). Moreover, we showed that extracellular matrix (ECM)
beta1-Integrin-collagen interaction reduces chondrocyte apoptosis
Cao L, et al.
Matrix Biology, 18(4), 343-355 (1999)
Sarah M Short et al.
The Journal of cell biology, 168(4), 643-653 (2005-02-18)
The anti-angiogenic effect of thrombospondin-1 has been shown to be mediated through binding of the type-1 repeat (TSR) domain to the CD36 transmembrane receptor. We now report that the TSR domain can inhibit VEGF-induced migration in human umbilical vein endothelial
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