推荐产品
生物源
rabbit
品質等級
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
產品線
Prestige Antibodies® Powered by Atlas Antibodies
形狀
buffered aqueous glycerol solution
物種活性
human
加強驗證
RNAi knockdown
Learn more about Antibody Enhanced Validation
技術
immunoblotting: 0.04-0.4 μg/mL
immunofluorescence: 0.25-2 μg/mL
immunohistochemistry: 1:1000-1:2500
免疫原序列
GAPREVVDPSGGWATLLESIRQAGGIGKAKLRSMKERKLEKKKQKEQEQVRATSQGGHLMSDLFNKLVMRHKGISGKGPGAGEGPGGAFARVSDSIPPLPPPQQPQVDEDEDDWES
運輸包裝
wet ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... WASH1(100287171)
一般說明
WASH complex subunit 1 (WASHC1) is located on human chromosome 9p24.3. It belongs to Wiskott-Aldrich Syndrome Protein and SCAR Homolog (WASH) family. WASHC1 protein has an actin binding domain.
免疫原
WASH complex subunit 1 recombinant protein epitope signature tag (PrEST)
應用
Anti-WASHC1 antibody produced in rabbit has been used:
- in the detection of WASH complex in Dictyostelium cells by immunofluorescence imaging.
- in the detection of WASH proteins in HeLa cells using confocal microscopy.
- in western blotting for the detection of WASH1 complexes in human leukemic Jurkat T cells.
生化／生理作用
WASH exists as multiple complex protein in HeLa and bovine cells. WASH proteins coordinate the migration of endosomal α5β1 integrin. WASHC1 protein regulates actin cytoskeleton organisation. WASH1 protein is regarded as actin-nucleating promoting factor. WASH protein complex interacts with actin related protein, Arp2/3 and mediates actin networking.
特點和優勢
Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
Every Prestige Antibody is tested in the following ways:
- IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
- Protein array of 364 human recombinant protein fragments.
聯結
Corresponding Antigen APREST74318
外觀
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
法律資訊
Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
The Arp2/3 activator WASH regulates alpha5beta1-integrin-mediated invasive migration
Zech T, et al.
Journal of Cell Science, 124(22), 3753-3759 (2011)
Functional characterization of Wiskott Aldrich Syndrome protein and scar homolog (WASH), a bi-modular nucleation promoting factor (NPF) able to interact with biogenesis of lysosome related organelle subunit 2 (BLOS-2) and gamma-tubulin
Monfregola J, et al.
The Journal of Biological Chemistry, jbc-M109 (2010)
Catherine M Buckley et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(40), E5906-E5915 (2016-09-21)
Macropinocytosis is an ancient mechanism that allows cells to harvest nutrients from extracellular media, which also allows immune cells to sample antigens from their surroundings. During macropinosome formation, bulk plasma membrane is internalized with all its integral proteins. It is
Human subtelomeric WASH genes encode a new subclass of the WASP family
Linardopoulou EV, et al.
PLoS Genetics, 3(12), e237-e237 (2007)
The cargo-selective retromer complex is a recruiting hub for protein complexes that regulate endosomal tubule dynamics
Harbour ME, et al.
The Journal of Biological Chemistry, jcs-071472 (2010)
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