推荐产品
包裝
pkg of 1 ea
製造商/商標名
Cytiva 28-9782-44
參數
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
床尺寸
7.7 mm × 100 mm
床體積
4.7 mL
柱內徑
7.7 mm
基質
6% cross-linked agarose
粒徑
45-165 μm
平均直徑
90 μm
cleaning
2-14(Ni2+-stripped medium.)
工作範圍
3-12(Ni2+-stripped medium.)
容量
~40 mg binding capacity(histidine-tagged protein)
適合性
suitable for bioprocess medium
一般說明
應用
HiScreen™ Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose™ 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.
特點和優勢
- High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
- Prepacked, ready-to-use columns for convenient process development.
- Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
儲存和穩定性
分析報告
法律資訊
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
商品
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
实验方案
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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