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Key Documents

GE28-9782-44

HiScreen Ni Fast Flow

Cytiva 28-9782-44

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About This Item

分類程式碼代碼:
13111000
NACRES:
NA.56

包裝

pkg of 1 ea

製造商/商標名

Cytiva 28-9782-44

參數

1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi

床尺寸

7.7 mm × 100 mm

床體積

4.7 mL

柱內徑

7.7 mm

基質

6% cross-linked agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

2-14(Ni2+-stripped medium.)

工作範圍

3-12(Ni2+-stripped medium.)

容量

~40 mg binding capacity(histidine-tagged protein)

適合性

suitable for bioprocess medium

一般說明

HiScreen columns prepacked with Ni Sepharose 6 Fast FLow for method optimization and parameter screening for purification of histidine-tagged proteins with Immobilized Metal ion Affinity Chromatography (IMAC).

應用

HiScreen columns are part of the process development platform available from Cytiva. The columns are prepacked with a range of BioProcess chromatography media (resin) and designed for method optimization and parameter screening. HiScreen columns have small bed volumes (4.7 ml), reducing the cost of sample and buffer consumption. The chromatography media used in HiScreen columns are also available in other prepacked formats and as bulk packs, for all scales of work from development and pilot studies to routine production.

HiScreen Ni FF is prepacked with the BioProcess chromatography medium Ni Sepharose 6 Fast FLow which has high binding capacities for
histidine-tagged Proteins and minimal Ni2+ leakage.

特點和優勢

  • High binding capacity, approx. 40 mg/mL medium and negligible leakage of Ni2+.
  • Prepacked, ready-to-use columns for convenient process development.
  • Excellent choice for method optimization and parameter screening due to the 10 cm bed height.
  • Easy connection in series to achieve 20 cm bed height.
  • Small bed volume gives fast results and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

HiScreen is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Environment

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


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商品

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

实验方案

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from GE Healthcare.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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