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材料
polycarbonate housing
polyethersulfone membrane
無菌
non-sterile
包裝
pack of 25 pieces
製造商／商標名
Cytiva 28-9322-48
存貨情形
not available in Japan
管尺寸
~15 mL
有效容積
2 mL
孔徑
30 kDa MWCO
儲存溫度
room temp
一般說明
Designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%.
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest
Vivaspin® sample concentrators are designed for fast, nondenaturing concentration of biological samples by membrane ultrafiltration. Up to 30-fold concentration of the sample can be achieved with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube with an upper compartment containing sample and lower compartment separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by the user. Centrifugation is applied to force solvent through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin® sample concentrators cater for sample volumes from 100 μl to 20 ml, with a range of molecular weight cutoff values from Mr 3 000 to 100 000. For maximum recovery, select a MWCO at least 50% smaller than the molecular size of the species of interest
特點和優勢
- Compatible pH range is from pH 1 to 9.
- Patented dead-stop technology, which ensures that samples cannot be concentrated to dryness and enables direct concentrate recovery
- One-step sample concentration in a single tube for minimal sample handling and reduced sample loss.
- Vertical polyethersulfone membrane minimizes membrane blockage and tolerates high flow rates.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Vivaspin is a registered trademark of Sartorius Stedim Biotech GmbH
儲存類別代碼
13 - Non Combustible Solids
商品
This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.
实验方案
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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