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ligand
recombinant protein G lacking albumin-binding region
材料
polypropylene and polyethylene
特點
wetted part: no
包裝
pkg of 4 ea
製造商／商標名
Cytiva 28-9031-35
儲存條件
(20% Ehtanol)
(20% Ethanol)
參數
30 °C max. temp.
4 °C min. temp.
尺寸
127.8 mm × 85.5 mm × 30.6 mm
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning
2-9
工作範圍
3-9
容量
>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
儲存溫度
2-8°C
一般說明
Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species.
Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).
A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
Protein G Sepharose™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).
A protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min is also provided. No pretreatment of the sample is required.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
特點和優勢
- Fast, reliable ease-of-use format for small-scale purification of antibodies or enrichment of proteins of interest in prepacked 96-well filter plates that are ready to use.
- Flexible format, protocol for classical elution as well as cross-linking method.
- Formatted for elution for electrophoresis or LC-MS analysis workflows.
- Fast binding kinetics and high capacity provides high yield.
- Easy scale-up with HiTrap® Protein G HP prepacked columns.
- Prepacked with Protein G Sepharose™ High Performance for coupling of antibodies of IgG subclasses.
- MultiTrap™ 96-well filter plates can be used with centrifugation or vacuum, manually or in automated robotic systems.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
HiTrap is a registered trademark of Cytiva
MultiTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
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This page describes immunoprecipitation (immunoaffinity or pull-down techniques).
Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.
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