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Key Documents

GE25-6400-50

TempliPhi 500 Amplification Kit

Cytiva 25-6400-50

别名:

rolling circle amplification (RCA), circular ssDNA/ dsDNA amplification

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About This Item

分類程式碼代碼:
41116006
NACRES:
NA.85

製造商/商標名

Cytiva 25-6400-50

運輸包裝

dry ice

儲存溫度

−70°C

一般說明

illustra TempliPhi DNA Amplification Kits use isothermal rolling circle amplification (RCA) to efficiently prepare DNA sequencing templates.

TempliPhi Kits use a unique process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30° C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.

Two different types of TempliPhi DNA Amplification Kits are available to meet different throughput requirements. illustra TempliPhi DNA Amplification Kit for 2000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability.

The illustra TempliPhi DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed.

應用

Preparation of circular DNA templates for cycle sequencing, cloning, and transformation.

特點和優勢

  • Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h.
  • Generate microgram quantities of template DNA from picogram amounts of starting material.
  • Use amplified DNA directly for cycle sequencing without purification.
  • Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA.
  • Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation.
  • For low-to medium-throughput, small DNA vector preparation.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
The product can be s-red for 2 months or less at -20°C. For s-rage longer than 2 months, s-re at -70°C Thaw components on ice and maintain at 0–4°C during handling. DO NOT warm above 4°C prior - amplification

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

TempliPhi
On packaging and insert for kits:
For use only as licensed by Qiagen GmbH. and Cytiva. The Phi 29 DNA polymerase may not be re-sold or used except in conjunction with the other components of this kit. See US patent numbers 5,854,033, 6,124,120, 6,143,495, 6,323,009, 5,198,543, 5,576,204, and their equivalents in other countries.
On container:
Subject to proprietary rights of Cytiva. May not be re-sold or used except with the other components of this kit. See US patent numbers 5,576,204, and their equivalents in other countries.
TempliPhi is a trademark of Cytiva
illustra is a trademark of Cytiva

相關產品

儲存類別代碼

12 - Non Combustible Liquids


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Michael J Reagin et al.
Journal of biomolecular techniques : JBT, 14(2), 143-148 (2003-12-17)
Preparing plasmid templates for DNA sequencing is the most time-consuming step in the sequencing process. Current template preparation methods rely on a labor-intensive, multistep procedure that takes up to 24 h and produces templates of varying quality and quantity. The
Tracy L Meiring et al.
Virology journal, 9, 164-164 (2012-08-18)
Human papillomavirus (HPV) is the aetiological agent for cervical cancer and genital warts. Concurrent HPV and HIV infection in the South African population is high. HIV positive (+) women are often infected with multiple, rare and undetermined HPV types. Data

实验方案

This page shows circular DNA amplification using TempliPhi™ from GE Healthcare.

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