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Key Documents

GE17-5442-03

Ficoll®-Paque Premium

Cytiva 17-5442-03, pack of 6 × 500 mL

别名:

Ficoll density gradient medium, cell culture media

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About This Item

分類程式碼代碼:
12352201
NACRES:
NA.25

形狀

liquid

包裝

pack of 6 × 500 mL

製造商／商標名

Cytiva 17-5442-03

顏色

Colorless to slightly yellow

pH值範圍

5.5-7.5

一般說明

Ficoll-Paque PREMIUM products are a range of sterile, ready-to-use density gradient media for the preparation of mononuclear cells.

Ficoll-Paque PREMIUM products are based on Ficoll-Paque PLUS (see Ficoll-Paque PLUS) which has been used for more than 30 yr as a sterile density medium for the isolation of high yields of mononuclear cells from bone marrow, peripheral blood, and cord blood. All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485:2003.

應用

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.

Can be used when isolating lower-density human mononuclear cells (e.g., mesenchymal stromal cells or monocytes).

特點和優勢

  • Sterile, ready to use reagents used to prepare mononuclear cell preparations from peripheral blood, bone marrow, and umbilical cord blood by density gradient centrifugation.
  • Manufactured under a Quality Management System certified to ISO 13485:2003.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C ()

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Ficoll is a registered trademark of Cytiva

儲存類別代碼

12 - Non Combustible Liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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商品

How to Fractionate and Analyze Gradients of Percoll

Density Marker Beads are dyed derivatives of Sephadex™. There are ten color-coded bead types, each with a specific density. They have been specifically formulated for use in Percoll gradients and will not work with other media. Using Density Marker Beads as an external marker facilitates monitoring of the gradient shape and range.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

Removal of Percoll after Centrifugation

This page shows methods to remove Percoll from a sample after centrifugation.

Applications of Percoll in Other Cell Types

The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.

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实验方案

How to make and use gradients of Percoll

How to use percoll to prepare a gradient

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