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無菌
sterile-filtered
品質等級
形狀
liquid
儲存期限
Expiry date on the label.
雜質
endotoxin, tested
密度
1.119
應用
hematology
histology
儲存溫度
2-8°C
一般說明
Histopaque®-1119 是一种无菌、经过内毒素测试的即用型培养基。该溶液由聚蔗糖和泛影酸钠组成,经调整密度为 1.119 g/mL,pH 为 8.8–9.0。
應用
Histopaque®-1119适合与Histopaque-1077一起使用,因为它有助于从少量全血或骨髓中快速恢复活性单个核细胞和粒细胞。
特點和優勢
Histopaque®-1119 有许多优势,比如:
- 可使活细胞极佳恢复
- 允许选择分离不同种类的血细胞
- 防止细胞变形
- 保持细胞活力
- 最小化外来细胞干扰
- 各批次性能可重复
- 经过过滤杀菌和内毒素检测
- 在 2-8°C 避光保护条件下可稳定保存至少2年
原則
该过程涉及在 Histopaque-1119 上使等体积的 Histopaque-1077 分层,形成双梯度。将抗凝全血小心地铺在上层 Histopaque-1077 培养基上。离心后,红细胞被聚蔗糖聚集并因此迅速沉淀。粒细胞位于下部Histopaque-1077/1119 界面; 而在上部血浆/Histopaque-1077 界面处有淋巴细胞和其它单核细胞。在上部界面中发现的大多数外部血小板在洗涤步骤中通过低速离心去除。红细胞污染可以忽略不计。
法律資訊
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
相關產品
产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2
儲存類別代碼
8A - Combustible corrosive hazardous materials
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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