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G8295

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于大肠杆菌

≥10,000,000 units/g protein (30 min assay), recombinant, expressed in E. coli overproducing strain, lyophilized powder

别名:

β-D-葡萄糖苷酸葡萄糖醛酸水解酶

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About This Item

CAS号:
9001-45-0
EC 号:
3.2.1.31 (BRENDA, IUBMB)
EC號碼:
232-606-8
MDL號碼:
MFCD00130629
分類程式碼代碼:
12352204
NACRES:
NA.54

重組細胞

expressed in E. coli overproducing strain

品質等級

300

形狀

lyophilized powder

比活性

≥10,000,000 units/g protein (30 min assay)

分子量

~290 kDa

成份

protein, 40-60% biuret

儲存溫度

−20°C

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應用

比较不同酶性能的新技术文章
了解更多
了解Sigma研发获得的最新应用数据，关于使用不同来源的酶优化不同药物类别的水解，并使用色谱纯化酶降低酯酶活性导致6-单乙酰吗啡(6-MAM)转化为吗啡的影响
有效水解类固醇葡糖苷酸。
用于尿液代谢物分析中葡糖苷酸结合物的水解

品質

含有缓冲盐和稳定剂。

單位定義

一个Sigma或改良的Fishman单元将在37℃，pH6.8（30分钟测定）下每小时从酚酞葡糖苷酸中释放1.0 μg酚酞。

象形圖

Health hazard
GHS08

訊號詞

Danger

危險聲明

H317,H334

危險分類

Resp. Sens. 1 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Enzymes
Methods of Enzymatic Analysis, 2, 460-461 (1974)
Ling Xiao et al.
RSC advances, 10(39), 22966-22971 (2020-06-16)
UDP-glucuronosyltransferases (UGTs) and β-glucuronidase (GUS) catalyze entirely distinct metabolism reactions. UGTs are responsible for the glucuronidation of a variety of drugs, endogenous and environmental chemicals, whereas GUS hydrolyzes glucuronides and liberates the parent substrates. Information on the overlap of ligand
Optimized conditions for the emzymatic hydrolysis of a-hydroxytriazolam-glucoronide in human urine.
Tsujikawa, K., et al.
Health Laboratory Science, 50, 286-289 (2004)
Pascale Fança-Berthon et al.
The Journal of nutrition, 151(7), 1802-1816 (2021-04-21)
Curcuminoids from turmeric rhizome have significant health benefits but low bioavailability. To assess the pharmacokinetics of a novel natural turmeric dried colloidal suspension compared with 4 other turmeric formulations (including a standardized extract) at their respective recommended dosages. Thirty healthy men
V Graef et al.
Clinical chemistry, 23(3), 532-535 (1977-03-01)
We determined the enzymic activity of beta-glucuronidase preparations from bovine liver, Helix pomatia, and Escherischia coli with steroid glucuronides and nonsteroid glucuronides as substrates. We also studied the effect of Na2SO4 on the enzymic hydrolysis of several substrates with the
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Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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实验方案

Using UHPLC/MS (TOF) for Detection of Drugs and Metabolites in Urine Following Optimized Enzymatic Hydrolysis Conditions

To optimize hydrolysis using β-glucuronidase, factors such as incubation time, temperature, hydrolysis pH, enzyme source, and enzyme concentration must be evaluated for each glucuronide metabolite to be analyzed.

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