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Merck
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主要文件

G7550

Sigma-Aldrich

Sephadex® G-75

Superfine

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
23151817
NACRES:
NA.56

品質等級

技術

buffer exchange: suitable

基質活性組

phase

膨潤

1 g swells to 12-15 mL

珠尺寸

20-50 μm

應用

life science and biopharma

相容性

Cytiva

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一般說明

Sephadex® G-75是成熟的凝胶过滤介质,用于分子量为>80,000的大生物分子的脱盐和缓冲液交换。
Sephadex®是葡聚糖与环氧氯丙烷交联制备的凝胶过滤介质。不同类型的Sephadex的交联度不同,因此其溶胀程度和分子分级范围也不同。Sephadex® G-75用于较大的分子。

應用

Sephadex® G-75是凝胶过滤介质,用于凝胶过滤色谱和蛋白质色谱。它已用于脱盐过程,以研究壳聚糖纳米颗粒(CSNPs)中L-天冬酰胺酶II(ASNase II)的生产、纯化和固定化。

法律資訊

Sephadex is a registered trademark of Cytiva

取代透過

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Engineering a Disulfide Bond in Recombinant Manganese Peroxidase Results in Increased Thermostability
Reading, N.S., and Aust, S.D.
Biotechnol. Progress, 16(3), 326-333 (2000)
Sofia R Pauleta et al.
Biochemistry, 43(35), 11214-11225 (2004-09-16)
The gene for pseudoazurin was isolated from Paracoccus pantotrophus LMD 52.44 and expressed in a heterologous system with a yield of 54.3 mg of pure protein per liter of culture. The gene and protein were shown to be identical to
John G Vontas et al.
The Biochemical journal, 362(Pt 2), 329-337 (2002-02-21)
A novel glutathione S-transferase (GST)-based pyrethroid resistance mechanism was recently identified in Nilaparvata lugens [Vontas, Small and Hemingway (2001) Biochem. J. 357, 65-72]. To determine the nature of GSTs involved in conferring this resistance, the GSTs from resistant and susceptible
Elham Bahreini et al.
Nanoscale research letters, 9(1), 340-340 (2014-08-13)
This paper describes the production, purification, and immobilization of l-asparaginase II (ASNase II) in chitosan nanoparticles (CSNPs). ASNase II is an effective antineoplastic agent, used in the acute lymphoblastic leukemia chemotherapy. Cloned ASNase II gene (ansB) in pAED4 plasmid was
Fakhra Amin et al.
Protein and peptide letters, 18(2), 210-218 (2010-11-09)
Cystatins are thiol proteinase inhibitors ubiquitously present in mammalian body and serve various important physiological functions. To purify and characterize Thiol protease inhibitor from buffalo brain and to compare its properties with respect to tissue and organ difference from other

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