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Merck

G6751

Sigma-Aldrich

α-甘油磷酸脱氢酶 来源于兔肌肉

Type I, ammonium sulfate suspension, 100-300 units/mg protein

别名:

sn-甘油-3-磷酸:NAD+ 2-氧化还原酶, sn-甘油-3-磷酸脱氢酶 来源于兔肌肉

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

rabbit muscle

品質等級

種類

Type I

形狀

ammonium sulfate suspension

比活性

100-300 units/mg protein

儲存條件

(Tightly closed)

技術

activity assay: suitable

顏色

white

異物活動

Lactic dehydrogenase, pyruvate kinase, aldolase, and glyceraldehyde-3-phosphate dehydrogenase ≤0.01%
Triosephosphate isomerase ≤0.02%

儲存溫度

2-8°C

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相关类别

一般說明

α-磷酸甘油脱氢酶是一种膜蛋白,是黄素连接的初级脱氢酶。

研究领域:细胞信号传导

應用

α-磷酸甘油脱氢酶(来源于兔肌肉)已用于:
  • 配制反应混合物检测甘油激酶活性
  • 证明NADH参与的区室化酶反应
  • L-墨角藻糖-1-磷酸醛缩酶的反应混合物检测

生化/生理作用

α-磷酸甘油脱氢酶催化L-α-磷酸甘油(Glp)氧化形成磷酸二羟丙酮(DHAP)和过氧化氢(H2O2)。该酶参与呼吸电子传递链、糖酵解和磷脂代谢。

單位定義

在 pH 7.4,25℃ 下,一单位酶每分钟将 1.0 μmole 磷酸二羟丙酮转化为 α-甘油磷酸酯。

外觀

溶于 3.2M(NH42SO4 和 0.1g/L EDTA 溶液中的结晶悬浮液,pH 6.0

準備報告

根据改良的 Fondy 法进行制备。

分析報告

缩二脲法测定蛋白

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说明
价格

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Structural studies on nicotinamide adenine dinucleotide-linked L-glycerol 3-phosphate dehydrogenase crystallized from rat skeletal muscle.
T P Fondy et al.
The Journal of biological chemistry, 243(11), 3148-3160 (1968-06-10)
Joanne I Yeh et al.
Proceedings of the National Academy of Sciences of the United States of America, 105(9), 3280-3285 (2008-02-26)
Sn-glycerol-3-phosphate dehydrogenase (GlpD) is an essential membrane enzyme, functioning at the central junction of respiration, glycolysis, and phospholipid biosynthesis. Its critical role is indicated by the multitiered regulatory mechanisms that stringently controls its expression and function. Once expressed, GlpD activity
Susan K Boehlein et al.
The Plant journal : for cell and molecular biology, 99(1), 23-40 (2019-02-13)
Cereal yields decrease when grain fill proceeds under conditions of prolonged, moderately elevated temperatures. Endosperm-endogenous processes alter both rate and duration of dry weight gain, but underlying mechanisms remain unclear. Heat effects could be mediated by either abnormal, premature cessation
Tiia Kittilä et al.
Chembiochem : a European journal of chemical biology, 17(7), 576-584 (2016-01-12)
Nonribosomal peptide synthetases (NRPSs) produce many important and structurally complex natural products. Because of their architectures, reprogramming NRPSs has long been attempted to access new bioactive compounds. However, detailed characterization of NRPS catalysis and substrate selectivity by adenylation (A) domains
Talia Miron et al.
European journal of medicinal chemistry, 45(5), 1912-1918 (2010-02-06)
Allylsulfides from garlic are chemopreventive agents. Entering cells they are expected to initially interact with glutathione. Accordingly, reaction mechanisms of the product, S-allylthio-glutathione, with model proteins and thiols were analyzed in cell free systems. With glutathionyl, cysteinyl or captopril representing

商品

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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