推荐产品
生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
2.5D.17, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~55 kDa
物種活性
rat, mouse, human
濃度
~2 mg/mL
技術
immunohistochemistry: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of A549 cells
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... GFI1(2672)
mouse ... Gfi1(14581)
rat ... Gfi1(24388)
一般說明
Monoclonal Anti-Growth Factor Independence-1 (GFI1) (mouse IgG1 isotype) is derived from the hybridoma 2.5D.17 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with rat GFI1. Growth Factor Independence 1 (GFI1) is a zinc-finger transcriptional repressor, that is located on human chromosome 1p22. Gfi1 is highly expressed in thymus while Gfi1B is the predominant factor expressed in spleen, and both are expressed in bone marrow. It has a repression domain named SNAG at the N-terminal.† The protein also has a zinc finger DNA binding domain that is homologous to the domain in Gfi1B. Both bind to same consensus DNA sequence
特異性
The antibody epitope resides within the GFI1 SNAG transcriptional-repression domain (amino acids 7-26).
免疫原
rat growth factor independence-1 (GFI1).
應用
Monoclonal Anti-Growth Factor Independence-1 (GFI1) antibody may be used in:
- immunoblotting
- immunohistochemistry
- EMSA (electrophoretic mobility shift analysis)
生化／生理作用
Growth factor independence-1 (Gfi1) plays a key role in hematopoiesis along with Gfi1B proteins. Cytokines that regulate innate immunity induce Gfi1 expression. Deficiency of this protein causes thymic and peripherial T lymphopenia and severe abnormalities in pre-T-cell development in mice. Mutations in this gene cause defects in T lymphocyte function and development in humans. Gfi1 autoregulates its expression in T lymphocytes.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
分析報告
The antibody may also be used in EMSA (electrophoretic mobility shift analysis).
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Loretta L Doan et al.
Journal of immunology (Baltimore, Md. : 1950), 170(5), 2356-2366 (2003-02-21)
T cell differentiation in the thymus is dependent upon signaling through the TCR and is characterized by the resulting changes in expression patterns of CD4 and CD8 surface coreceptor molecules. Although recent studies have characterized the effects of proximal TCR
Targeted transcriptional repression of Gfi1 by GFI1 and GFI1B in lymphoid cells
Doan LL, et al.
Nucleic Acids Research, 32(8), 2508-2519 (2004)
Kazanjian, A., et al.
Cancer Research, 64, 68774-68782 (2004)
Prognostic significance of high GFI1 expression in AML of normal karyotype and its association with a FLT3-ITD signature
Volpe G, et al.
Scientific reports, 7(1), 11148-11148 (2017)
The downregulation of GFI1 by the EZH2-NDY1/KDM2B-JARID2 axis and by human cytomegalovirus (HCMV) associated factors allows the activation of the HCMV major IE promoter and the transition to productive infection
Sourvinos G, et al.
PLoS Pathogens, 10(5), e1004136-e1004136 (2014)
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