推荐产品
應用
Flourotag FITC 偶联试剂盒可以用于流式细胞仪免疫组织化学和免疫细胞化学。它还可以用于FITC和多肽激素，细胞活素，生长因子和蛋白质偶联。
特點和優勢
- 适于少量(1 mg)和大量(5 mg)偶联物。
- 完整水相法 - 不需要DMF
- 从过量的FITC快速凝胶过滤分离偶联物
- 偶联物和F/P比测定完整实验方案
- 试剂足够至少进行5次5mg蛋白质的偶联反应，可供在扩大化前确定最佳F/P比。
- 应用和实验方案参考
原則
Sigma提供方便的试剂盒用于制备FITC-标记抗体。异硫氰基荧光素（FITC），异构体1，是一种广泛使用的荧光团，因为在共轭反应时它有高量子效率和稳定性。FITC具有黄橘色的颜色以及在495 nm处的最大吸光值。通过激发，它可以散发出在525 nm具有最大发射的黄绿色。通过蛋白质或多肽链的自由氨基基团发生共轭反应，形成稳定的硫脲键（看反应）。FITC和抗体，凝集素，激素和生长因子共轭，用于各种免疫组织化学和流式细胞仪实验中。该实验方案对于抗体是最优方案，但用于其他蛋白质时需要用户自行修改。
分析報告
过程
1.在碳酸盐-重碳酸盐缓冲液中溶解蛋白质和FITC。
2.边搅拌边缓慢将FITC加入蛋白质。盖上箔片，在室温下搅拌2小时。
3.在G-25柱上将自由FITC和偶联物分开。收集组分。
4.收集含有偶联物的组分。
5.分光光度法确定F/P偶联物比例。
6.用1%牛血清白蛋白和0.1%叠氮钠稳定，0-5℃储存。
1.在碳酸盐-重碳酸盐缓冲液中溶解蛋白质和FITC。
2.边搅拌边缓慢将FITC加入蛋白质。盖上箔片，在室温下搅拌2小时。
3.在G-25柱上将自由FITC和偶联物分开。收集组分。
4.收集含有偶联物的组分。
5.分光光度法确定F/P偶联物比例。
6.用1%牛血清白蛋白和0.1%叠氮钠稳定，0-5℃储存。
法律資訊
FluoroTag is a trademark of Sigma-Aldrich Co. LLC
仅试剂盒组分
产品编号
说明
- Sephadex G-25 column, 3.5 mL 1
- Sephadex G-25 column, 9.1 mL 1
- Fluorescein isothiocyanate isomer I - F7250KC-2MG 2 mg
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- P3813Phosphate buffered saline, powder, pH 7.4, for preparing 1 L solutions 5 pkg化学品安全说明书
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
H Hidaka et al.
Journal of lipid research, 40(6), 1131-1139 (1999-06-05)
We previously reported the identity and purification of two HDL3-binding proteins in rat liver plasma membranes. As these proteins are candidate high density lipoprotein (HDL) receptors and probably multifunctional, including a role in HDL metabolism, we have considerable interest in
P Lloyd-Evans et al.
Transfusion medicine (Oxford, England), 9(2), 155-160 (1999-06-03)
The use of flow cytometry for quantifying fetomaternal haemorrhage is increasing, and has been shown to be more accurate than the Kleihauer-Betke test for evaluating larger bleeds of over 4 mL in volume. Red cells are stained with fluorescently labelled
Y Kobayashi et al.
Molecular human reproduction, 3(2), 91-99 (1997-02-01)
Leiomyomas are tumours of uterine smooth muscle tissue that are oestrogen and progesterone dependent. When explants of these tumours were grown in culture, the proliferating tissue formed characteristic ball-like aggregates (BLA), rather than the usual hill and valley (HV) pattern
R F Mullins et al.
Ophthalmology, 104(2), 288-294 (1997-02-01)
Drusen are extracellular deposits that accumulate between the basal lamina of the retinal pigment epithelium and the elastic lamina of Bruch membrane in aging human eyes. Although specific types of drusen are recognized as significant risk factors for the development
Extragenomic actions of progesterone in human sperm and progesterone metabolites in human platelets.
P F Blackmore
Steroids, 64(1-2), 149-156 (1999-05-14)
Progesterone rapidly increased intracellular free calcium ([Ca2+]i) in human sperm, removal of extracellular Ca2+ prevented the increase in [Ca2+]i. The Ca2+ influx was not blocked by the T-type Ca2+ channel blocker mibefradil. However T-type calcium channels do appear to be
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门