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Merck

F9012

Sigma-Aldrich

抗-山羊IgG(全分子)–FITC 兔抗

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

共軛

FITC conjugate

抗體表格

IgG fraction of antiserum

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

技術

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:160
indirect immunofluorescence: 1:200

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

The immunoglobulin G (IgG) is a major class of immunoglobulin and is highly conserved. IgG constitutes to 10−20% of the plasma proteins. This class of immunoglobulins are glycoproteins containing 82−96% protein and the remaining, carbohydrate. It exists in four subclasses namely, IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains containing two heavy and two light chains, linked by disulfide bonds.

特異性

The antiserum is determined to be immunospecific for goat IgG.

應用

Anti-Goat IgG (whole molecule)-FITC antibody produced in rabbit has been used for immunocytochemistry of human oocytes and preimplantation embryos.
It has also been used in enzyme linked immunosorbent assay (ELISA) and confocal microscopy.

生化/生理作用

Deficiency of a particular IgG subclass, may increase the susceptibility towards certain pathogens.
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Goat IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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