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一般說明
嗜热脂肪芽孢杆菌磷酸果糖激酶（BsPFK）是一种可被磷酸烯醇丙酮酸（PEP）沿着一个二聚体-二聚体界面结合而变构抑制的同四聚体。
来自嗜热脂肪芽孢杆菌的果糖6-磷酸激酶是一种34 kDa的酶，属于磷酸果糖激酶（PFK）样超家族。位于活性位点的谷氨酸161和精氨酸162残基对于底物结合至关重要。
應用
来自嗜热脂肪芽孢杆菌的果糖-6-磷酸激酶已显示出可与神经元一氧化氮合酶（nNOS）相互作用，引起糖酵解代谢障碍和营养不良性肌肉的易疲劳性增加。
来自嗜热脂肪芽孢杆菌的果糖-6-磷酸激酶已被用于：
- 在C8位氘化的ATP（C8-D ATP）存在下对果糖磷酸激酶活性进行稳态分析
- 定量肌肉磷酸果糖激酶（PFK）活性的标准曲线生成
Fructose-6-phosphate Kinase from Bacillus stearothermophilus has been used in the assay mixture for mass spectrometry assay for phosphoglucoisomerase (PGI) (G6P to F6P reaction).
生化／生理作用
果糖-1,6-二磷酸酶（FBP）是葡萄糖代谢中一种重要的酶。它可催化果糖-1,6-双磷酸水解为果糖6-磷酸和无机磷酸。在糖酵解循环的限速步骤中，果糖-6-磷酸激酶会将果糖-6-磷酸转化为果糖1,6-双磷酸。
Phosphofructokinase (PFK) is an essential bifunctional enzyme that serves as a critical regulator in the intermediate stages of glycolysis. PFK is strongly linked to caveolae and is brought to caveolae by caveolin-1 in vascular smooth muscle cells (VSMCs).
單位定義
在pH 9.0、30 °C条件下，一单位在每分钟可将1.0 μm的果糖-6-磷酸和ATP转化为果糖1,6-二磷酸和ADP。
外觀
含缓冲盐的冻干粉末（如磷酸盐缓冲液或含NaCl的Tris缓冲液）
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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