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Merck

E1031

Sigma-Aldrich

抗ERGIC-53/p58 兔抗

affinity isolated antibody, buffered aqueous solution

别名:

抗ERGIC53, 抗F5F8D, 抗FMFD1, 抗LMAN1, 抗MCFD1, 抗MR60, 抗gp58

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen ~58 kDa

物種活性

mouse, rat, human

技術

indirect immunofluorescence: 5-10 μg/mL using human HepG2 or rat NRK cells
western blot (chemiluminescent): 0.5-1.0 μg/mL using whole cell extract of mouse NIH3T3 cells

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... LMAN1(3998)
mouse ... Lman1(70361)
rat ... Lman1(116666)

一般說明

ERGIC(ER-高尔基体中间室)与I型膜标记蛋白ERGIC-53有关,其大鼠同源物称为(p58)。 ERGIC 是一种动态膜系统,由靠近 ER 出口位点的管状囊泡簇组成,有助于蛋白质从 ER 转运至高尔基体。抗ERGIC-53/(p58)抗体可用作一抗(1:125稀释),适用于荧光显微镜。它还可用于蛋白印迹分析。兔抗 ERGIC-53/ (p58) 抗体与大鼠、小鼠和人的 ERGIC-53/ (p58) 发生特异性反应。

免疫原

对应于大鼠p58的氨基酸158-170的合成肽,N-末端添加半胱氨酸,与KLH偶联。与小鼠和人的相应序列相同。

應用

ERGIC-53/(p58) 抗体可用于免疫印迹和免疫荧光分析。

外觀

溶于0.01M磷酸盐缓冲盐水,pH 7.4,含有1%牛血清白蛋白以及15 mM叠氮化钠。

免責聲明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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A Schweizer et al.
The Journal of cell biology, 107(5), 1643-1653 (1988-11-01)
Purified Golgi membranes of the human intestinal adenocarcinoma cell line Caco-2 were used as an antigen to produce a monoclonal antibody, G1/93, which specifically labels a tubulovesicular compartment near the cis side of the Golgi apparatus, including the first cis-cisterna
AT-1 is the ER membrane acetyl-CoA transporter and is essential for cell viability.
Mary Cabell Jonas, Mariana Pehar
Journal of Cell Science, 23, 3378-3388 (2010)
Yu Lan et al.
Development (Cambridge, England), 143(13), 2344-2355 (2016-05-27)
Cleft palate is a common major birth defect for which currently known causes account for less than 30% of pathology in humans. In this study, we carried out mutagenesis screening in mice to identify new regulators of palatogenesis. Through genetic
Adam K Walker et al.
PloS one, 8(11), e81170-e81170 (2013-12-07)
In amyotrophic lateral sclerosis (ALS) and frontotemporal lobar degeneration, TAR DNA binding protein 43 (TDP-43) accumulates in the cytoplasm of affected neurons and glia, where it associates with stress granules (SGs) and forms large inclusions. SGs form in response to
J Saraste et al.
The Journal of cell biology, 105(5), 2021-2029 (1987-11-01)
A 58-kD cis-Golgi protein has been identified by generating polyclonal antibodies against heavy (cis) Golgi subfractions. Total microsomes isolated from rat pancreatic homogenates were subfractionated to yield a rough microsomal fraction (B1) and three smooth membrane subfractions (B2-B4) enriched in

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