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Merck

D9154

Sigma-Aldrich

邻联茴香胺 二盐酸盐

tablet

别名:

3,3'-二甲氧基联苯胺 二盐酸盐, 固蓝 B

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About This Item

经验公式(希尔记法):
C14H16N2O2 · 2HCl
CAS号:
分子量:
317.21
Beilstein:
3917996
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.83

product name

邻联茴香胺 二盐酸盐, tablet, 10 mg substrate per tablet

形狀

tablet

溶解度

water: 1 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI 密鑰

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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相关类别

特異性

邻联茴香胺(3,3′-dimethoxybenzidine)是一种适合在 ELISA 步骤中使用的过氧化物酶底物。该底物产生可溶性终产物,颜色为黄橙色,可在 405 nm 处用分光光度法读取。可用 5 MHCl 终止反应。

警告

可能致癌。

重構

Dissolve one tablet in 60 ml of 50 mM phosphate-citrate buffer, pH 5.0. Add 12 μl of fresh 30% hydrogen peroxide immediately before to use.

訊號詞

Danger

危險聲明

危險分類

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges


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商品

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

实验方案

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay of Glucose Oxidase

This procedure applies to products that have a specification for the enzymatic activity of cholesterol oxidase. This assay is NOT to be used to assay Cholesterol Oxidase from Schizophyllum commune (Discontinued Product Number C7274) and from Brevibacterium sp. (Discontinued Product Number C8153).

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