推荐产品
生物源
bacterial (Leuconostoc mesenteroides)
形狀
solid
運輸包裝
wet ice
儲存溫度
−20°C
應用
The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. This standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide, dependent upon the chromatographic conditions employed. The elution position of each peak in this ladder is expressed as a glucose unit (gu) and is used to assign gu values to peaks in the released glycan pool.Dextran ladder upon derivitization can be used as a new tool to assess cell layer tightness.
準備報告
The dextran ladder is prepared by partial acid hydrolysis of dextran from Leuconostoc mesenteroides with an average molecular weight of 100-200 kDa. The purity and structural integrity of the ladder is assessed by fluorescently labeling an aliquot and subsequent analysis by normal phase HPLC.
相關產品
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
其他客户在看
A novel tool to characterize paracellular transport: the APTS-dextran ladder.
Neuhaus W, Bogner E, Wirth M, et al.
Pharmaceut. Res., 23, 1491-1501 (2006)
Winfried Neuhaus et al.
Journal of pharmaceutical and biomedical analysis, 40(4), 1035-1039 (2005-10-26)
The aim of this work was the development of an easy manageable analytic system for describing tightness of cell layers in a molecular size dependent manner, which is more precise than currently used ones. Dextrans were labeled by reductive amination
J Charlwood et al.
Analytical biochemistry, 273(2), 261-277 (1999-09-02)
Reverse- and normal-phase chromatography have been used to separate a number of standard human milk oligosaccharides derivatized via a reductive amination reaction with 2-aminoacridone (2-AMAC). Analytes were detected by spectrofluorimetry and injected simultaneously with a hydrolyzed dextran ladder derivatized with
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