推荐产品
一般說明
Sigma 公司的 DirectLoad ™ 50 bp DNA Step Ladder 包含 17 个平端片段,由 50~500 bp 的 50 bp 重复序列、600~900 bp 的 100 bp 重复序列和 1~3kb 的 1kb 重复序列组成。可以在琼脂糖或聚丙烯酰胺凝胶上的单一泳道中直接上样 10ul 的梯子。
應用
DirectLoad™ 50 bp DNA Step Ladder 已用于细胞裂解和基因组剪切检测分析。
适于通过琼脂糖或聚丙烯酰胺凝胶测定PCR产生的DNA片段的大小。
特點和優勢
- 可直接上样
- 易于使用
- 常见的条带大小
成分
5% 甘油、4.2 mM EDTA、0.083% 橙黄 G 和 0.0125% 二甲苯蓝的凝胶上样溶液中提供了 Sigma 的 DirectLoad ™ 50 bp DNA 梯形条带。
其他說明
为获得最佳分离度,推荐琼脂糖凝胶浓度为 2.0%。
法律資訊
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
其他客户在看
Solid-phase reverse transfection for intracellular delivery of functionally active proteins
Genome Research, 27(10), 1752-1758 (2017)
FEMS microbiology letters, 307(2), 158-164 (2010-04-24)
Culturable vibrios were isolated from seawater collected during an annual sampling study performed along the Gulf of Trieste coast (Northern Adriatic Sea), and conventional culturing and identification methods were used to investigate the presence of Vibrio parahaemolyticus. Biochemically selected Vibrio
商品
We offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments and RNA and can be concurrently run with the samples. All the markers stain well with common nucleic acid stains.
实验方案
JumpStart™ Taq DNA Polymerase is an antibody-inactivated, hot start enzyme.
Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.
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