推荐产品
用途
sufficient for 50 reactions (1 mL)
sufficient for 500 reactions (100 μL)
品質等級
運輸包裝
wet ice
儲存溫度
2-8°C
基因資訊
human ... NAGLU(4669)
應用
该试剂盒可在细胞溶解液、组织匀浆或纯化酶等样品中方便、快速地检测β-N-乙酰氨基葡萄糖苷酶。它是一种理想的分离细胞/组织样品溶酶体检测工具。
生化／生理作用
哺乳类动物体内的N-乙酰-D-氨基葡萄糖苷酶 (NAG) 是一种参与胞内糖脂和糖蛋白降解的溶酶体酶。在人类肾脏、肺和肝脏溶酶体中都已检测到高活性的NAG。血清NAG水平升高与某些癌症有关。
分析報告
该试剂盒的检测原理为NAG（NP-GLcNAc）底物在酶促作用下水解。酶水解底物后，释放出对硝基苯胺离子。然后在405 nm处采用比色法测定反应产物。
仅试剂盒组分
产品编号
说明
- Dilution Buffer 8 mL
- 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
- Citrate Buffer Solution, 0.09 M 100 mL
- p-Nitrophenol Standard Solution, 10 mM 1 mL
- β-N-Acetylglucosaminidase from Jack beans 1 vial
- Sodium carbonate 5 g
相關產品
訊號詞
Warning
危險聲明
危險分類
Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral
標靶器官
Liver,Kidney
儲存類別代碼
11 - Combustible Solids
其他客户在看
Richard A Zager et al.
Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association, 28(6), 1455-1462 (2013-04-02)
Recent experimental work suggests a paradox: although uremia evokes systemic toxicities, in the setting of AKI, it can induce intrarenal cytoprotective and anti-inflammatory effects. Whether these influences can attenuate post-ischemic kidney disease progression remains unknown. To explore this possibility, male
Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Xiaojia Liu et al.
Acta pharmaceutica Sinica. B, 11(10), 3134-3149 (2021-11-09)
Programmed cell death ligand 1 (PD-L1)/programmed cell death protein 1 (PD-1) cascade is an effective therapeutic target for immune checkpoint blockade (ICB) therapy. Targeting PD-L1/PD-1 axis by small-molecule drug is an attractive approach to enhance antitumor immunity. Using flow cytometry-based
Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of
商品
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门