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Merck

CLLS1059

Sigma-Aldrich

MCF10A CELLS BCR (-/-)

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32190102
NACRES:
NA.81

product name

MCF10A CELLS BCR (-/-),

生物源

human female mammary glands (Source Disease: Fibrocystic Disease)

OMIM登錄號

儲存溫度

−196°C

基因資訊

human ... BCR(613)

一般說明

MCF10A CELLS BCR (-/-) are pre-neoplastic mammary epithelial cells from a human adult female with a ZFN knock out modification.
This cell line corresponds to ATCC Catalog No. CRL-10317.

應用

Cell freezing medium-DMSO 1X, Cat. No. C6164
Media renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°
The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat No. H6909, to a final concentration of 0.5 ug/mL.

特點和優勢

These MCF10A cells are adherent with a doubling time of approx. 20 hours
Zinc finger nuclease (ZFN) knockout on chromosome 22q11

品質

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

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产品编号
说明
化学品安全说明书

  • MCF10A CONTROL CELLS 1 vial

  • MCF10A CELLS BCR (-/-) 1 vial


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We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.

We have applied our revolutionary CompoZr Zinc Finger Nuclease technology to create an unparalleled range of genetically modified mammalian cell lines for use in areas, such as target validation, drug discovery and drug development.

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