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CHP1

Sigma-Aldrich

Imprint® RNA免疫沉淀试剂盒

Complete ChIP reaction in 6 hours in flexible strip well format

别名:

Chromatin Immunoprecipitation

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About This Item

分類程式碼代碼:
41116158
NACRES:
NA.54

儲存溫度

−20°C

相关类别

一般說明

印记染色质免疫沉淀试剂盒为染色质免疫沉淀提供了完整的解决方案，包括用于DNA纯化的色谱柱和试剂，以及使用我们的GenomePlex® 全基因组扩增试剂盒（WGA2）进行ChIP DNA扩增的整体实验方案。灵活的带孔形式可以允许从哺乳动物细胞或组织中对DNA进行免疫沉淀和纯化。

有关此试剂盒的常见问题，请参阅 ChIP疑难解答。

應用

印迹染色质免疫沉淀试剂盒是一种灵活、快速且完整的方法，可用于通过染色质免疫沉淀（ChIP）研究蛋白质与DNA的相互作用。
  • 适用于下游应用
  • 从单个靶标表征到全基因组分析的技术
  • 信号转导途径的表征
  • ChIp-chIP和ChIP-seq数据的验证

特點和優勢

  • 快速—总实验操作时间少于6小时，使得Imprint试剂盒成为市场上最快速的选择
  • 灵敏—每个ChIP样品仅需要10,000个细胞
  • 方便—在目前所有ChIP实验方案中其步骤是最少的
  • 灵活—可用于细胞或组织的实验方案中，以及提供可用于高通量应用的便捷条带孔形式
  • 完整—包括用于DNA纯化的色谱柱和试剂，以及用于通过GenomePlex技术进行扩增的整体实验方案

法律資訊

GenomePlex is a registered trademark of Takara Bio USA, Inc.
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL化学品安全说明书
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution化学品安全说明书

訊號詞

Danger

危險分類

Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Central nervous system

儲存類別代碼

3 - Flammable liquids

閃點（°F）

53.6 °F - closed cup

閃點（°C）

12 °C - closed cup

分析证书(COA)

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Ghazala Begum et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 26(4), 1694-1703 (2012-01-10)
Undernutrition during pregnancy is implicated in the programming of offspring for the development of obesity and diabetes. We hypothesized that maternal programming causes epigenetic changes in fetal hypothalamic pathways regulating metabolism. This study used sheep to examine the effect of
Poulomi Banerjee et al.
Journal of molecular medicine (Berlin, Germany), 94(12), 1397-1409 (2016-08-19)
Epithelial-mesenchymal transition (EMT) is one of the key biological phenomena behind cancer and metastasis. Clinical studies suggest that patients undergoing metformin therapy are less predisposed to cancer but the underlying mechanism is far from clear. Given that metformin also acts
Lucie Hyrsova et al.
British journal of pharmacology, 173(10), 1703-1715 (2016-02-28)
The organic cation transporter 1 (OCT1) transports cationic drugs into hepatocytes. The high hepatic expression of OCT1 is controlled by the HNF4α and USF transcription factors. Pregnane X receptor (PXR) mediates induction of the principal xenobiotic metabolizing enzymes and transporters
Thomas Palm et al.
Nucleic acids research, 41(6), 3699-3712 (2013-02-12)
Stem cell fate decisions are controlled by a molecular network in which transcription factors and miRNAs are of key importance. To systemically investigate their impact on neural stem cell (NSC) maintenance and neuronal commitment, we performed a high-throughput mRNA and
Ulrich Deuschle et al.
International journal of cancer, 136(11), 2693-2704 (2014-11-05)
The nuclear bile acid receptor Farnesoid X receptor (FXR) is strongly expressed in liver and intestine, controls bile acid and lipid homeostasis and exerts tumor-protective functions in liver and intestine. Histidine-rich glycoprotein (HRG) is an abundant plasma protein produced by
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实验方案

ChIP-qPCR Data Analysis

Chromatin Immunoprecipitation qPCR for studying gene regulation across conditions.

Imprint® Chromatin Immunoprecipitation Kit (CHP1) Protocol

The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format

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