Ri-1

96090512

• 分類程式碼代碼: 41106514

属性

• 生物源: human blood
• 增長模式: Suspension
• 染色體組型: 92XXXX/69XXX,t(1,2)(p32,q34)t(2,8)(p11,q24)t(14,18
• 形態學: Lymphoblastoid
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian B-cell lymphoma

細胞系描述

The cell line Ri-1 has been established from the peripheral blood of a 48-year-old female patient with B-cell lymphoma. This EBV-negative cell line with 14q+ abnormality was shown to be at an intermediate stage of B-cell differentiation. Ri-1 expresses surface and cytoplasmic immunoglobulin (IgM kappa), HLA-DR, CD20, CD38 and FMC7. Rearranged heavy-light chain genes and one rearranged kappa light-chain gene was detected as well as a kappa and lambda light-chain gene in germ-line configuration.

應用

Study of B-cell lymphoma-leukaemia

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth is very slow after resuscitation; large amounts of debris present.

其他說明

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