M15

95102517, mouse kidney, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: M15, 95102517
• 生物源: mouse kidney
• 增長模式: Adherent
• 染色體組型: Hyperdiploid
• 形態學: Epithelial
• 產品: WT1 (expressed gene)
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse mesonephric epithelium, polyoma virus large T transformed

細胞系描述

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

應用

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

其他說明

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