Hepa-1c1c7

95090613, mouse liver, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: Hepa-1c1c7, 95090613
• 生物源: mouse liver
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Aryl hydrocarbon
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse hepatoma

細胞系描述

Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.

應用

Study of Ah receptors, P450IA1 regulation

培養基

α MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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