PNT1A human

95012614, human prostate, Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: PNT1A human, 95012614, Normal prostate epithelium imortalized with SV40.
• 生物源: human prostate
• 增長模式: Adherent
• 染色體組型: Aneuploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Dihydrotestosterone receptor
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −20°C

说明

細胞系來源

Human post pubertal prostate normal, immortalised with SV40

細胞系描述

Established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from the prostate of a 35 year old male at post mortem. The cells contain the SV40 genome and express large T protein. They present the phenotype of differentiated luminal prostatic cells with the expression of cytokeratin 8 and 18 (markers for luminal glandular epithelia) and vimentin. Cytokeratin14, a marker of epithelial basal cells, is not expressed. PNT1A cells are non-tumourigenic in nude mice.

應用

PNT1A has been used to determine the variation in metabolite levels of human DU145 prostate cancer and PNT1A cells after treatment with selenomethionine (SeM) and Se-methylselenocysteine (SeMSC). It has also been used to study the effects of angiotensin II (Ang II) and relaxin 2 (RLN2) on the viability, proliferation, adhesion, migration and invasion of PNT1A cells.

Study of proliferation, oncogene activation and differentiation of adult prostatic cells and initial steps leading to transformation.

培養基

RPMI 1640 + 2mM Glutamine + 6 -10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37°C.

其他說明

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