G

94101453, rat prostate, Epithelial

• 分類程式碼代碼: 41106514

属性

• 产品名称: G, 94101453
• 生物源: rat prostate
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Androgen sensitive
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Dunning R-3327,prostate adenocarcinoma

細胞系描述

Derived from a Dunning R-3327 rat with prostatic adenocarcinoma. G is one of a group of variants which developed by a single in vivo passage. It is androgen responsive, moderately fast growing and poorly differentiated. Using this system of rat prostatic adenocarcinomas as a model, it has been demonstrated that prostatic cancer cells can become unstable at any time resulting in the addition of cytogenetically and phenotypically novel clones of cancer cells to the cancer population.

培養基

RPMI 1640 + 2mM Glutamine + 250nM Dexamethasone (DXMT) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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