VA-ES-BJ

94092303, human skin (bone marrow metastasis), Epithelial

• 分類程式碼代碼: 41106514

属性

• product name: VA-ES-BJ, 94092303
• 生物源: human skin (bone marrow metastasis)
• 增長模式: Adherent
• 染色體組型: 66-68 Aneuploidic, pentasomy #7, multiple trisomy
• 形態學: Epithelial
• 產品: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF)
• 受體: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), AE, AE3, Fas, epithelial membrane antigen (EMA), Vimentin, P53
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human skin epithelioid sarcoma

細胞系描述

Spontaneously transformed cells from a male patient with epithelioid sarcoma derived from bone marrow metastasis. Cells grow very rapidly and secrete GM-CSF. They have been shown to express AE-1 and AE-3 cytokeratins, epithelial membrane antigen, p53, human Fas and vimentin. VA-ES-BJ cells are sensitive to oxidising agents, busulfaxamine and protoporphyrin IX. Epithelial, spindle and giant cells.

應用

Study of tumours. Tumourigenic in nude mice.

培養基

DMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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