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生物源
mouse nerve
包裝
tube of 5 μg 93120817-DNA-5UG
pkg of vial of cells 93120817-1VL
增長模式
Adherent
染色體組型
Not specified
形態學
Neuronal-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse neuroblastoma
細胞系描述
CLONE NA is a subclone of Neuro-2a, a clonal line of C1300 mouse neuroblastoma cells. The cells were selected for spontaneous mutations deficient in HGPRT in the presence of 8-azaguanine. This cell line has been mycoplasma eradicated at ECACC.
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cultures may not form a complete monolayer before subculture is required.
其他說明
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