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产品名称
WIL2.NS.6TG, 93031001
生物源
human spleen
增長模式
Suspension
染色體組型
Not specified
形態學
Lymphoblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human Caucasian B lymphocyte
細胞系描述
A non Ig-secreting, HAT sensitive, 6-thioguanine resistant, human B lymphocyte. Derived from the parent line WIL2.NS (Sigma Catalogue number. 90112121).
應用
Immortalising antigen-stimulated human B-lymphocytes by somatic hybridisation
培養基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Maintain cultures between 5x1,000-5x10,000 cells/ml; 5% CO2; 37°C.
其他說明
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