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生物源
human kidney
增長模式
Adherent
染色體組型
2n = 46, aneuploid
形態學
Epithelial
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Human kidney adenocarcinoma
細胞系描述
Derived from 78-year-old male. Does not produce tumours in nude mice.
應用
Tumourigenicity studies
培養基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-2x100,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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