ND7/23 Cell Line

92090903, Mouse x Rat Hybridoma nerve, Neuronal

• 别名: ND7/23 Cells
• 分類程式碼代碼: 41106514

属性

• product name: ND7/23 Cell Line, 92090903, Mouse neuroblastoma X Rat neuron hybrid
• 生物源: Mouse x Rat Hybridoma nerve
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse neuroblastoma x Rat neurone hybrid

細胞系描述

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

應用

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x10⁴ viable cells/cm² at 37°C and 5% CO₂. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

其他說明

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