293 N3S

92052131

• 分類程式碼代碼: 41106514

属性

• 生物源: human kidney
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Adenovirus 5 early region (E1) antigens
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human embryo kidney

細胞系描述

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

培養基

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

例行更新培養

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

其他說明

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