G-402

90112715

• 分類程式碼代碼: 41106514

属性

• 生物源: human kidney
• 增長模式: Adherent
• 染色體組型: Modal no. 47
• 形態學: Epithelial-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Caucasian renal leiomyoblastoma

細胞系描述

Derived from a tumour of a 9 month old female Caucasian. Highly transformed and grows in soft agar.

應用

Transformation and tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,12
D16S539: 11,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,9
TPOX: 10
vWA: 13,17

培養基

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Change medium every 4 days.

其他說明

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