M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• 分類程式碼代碼: 41106514

属性

• product name: M-MSV-BALB/3T3, 90030802
• 生物源: mouse embryo
• 增長模式: Adherent
• 染色體組型: Modal no. 57
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

細胞系描述

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

應用

Transformation and virus studies: M-MSV, MuLV

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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