Y13-259

89111412, Reactivity: Anti-p21 Protein, Harvey Murine Sarcoma Virus

• 分類程式碼代碼: 41106514

属性

• 生物源: Rat x Rat Hybridoma
• ImmunoDon: Osborne-Mendel rat spleen
• 骨髓瘤: Y3Ag1.2.3
• 反應: Anti-p21 Protein, Harvey Murine Sarcoma Virus
• 技術: cell culture | mammalian: suitable
• 同型: IgG1
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat Y3Ag1.2.3 x Rat Osborne-Mendel spleen

細胞系描述

The antibody reacts to p21 encoded by Ha-MuSV v-ras gene. Cross-reacts to p21 encoded by Kirsten MuSV v-ras gene, p21 encoded by BALB-SV and 29kDa gag-ras fusion protein encoded by Ra-SV. Recognises p21 species in some uninfected rodent, carnivore and primate lines.

免疫原

Ha-MuSV transformed syngeneic NRK cells

培養基

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells will semi-attach. Shake cells off or use trypsin to remove cells from flask

其他說明

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