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产品名称
A-72, 89050908
生物源
dog thigh
增長模式
Adherent
染色體組型
Not specified
形態學
Fibroblast-like
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Canine Golden Retriever tumour
細胞系描述
Derived from tumour of thigh (unknown histology) of 8 year old Golden Retriever which had a previous history of mammary adenocarcinoma. Susceptible to canine corona-, canine Ad type I and II, canine herpes, canine parainfluenza and canine parvo- viruses.
應用
Virus studies: Canine corona-, Ad I and II, parainfluenza, herpes and parvo- viruses
培養基
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 0.02% EDTA; 37°C. CO2 is detrimental; free gas exchange with atmospheric air necessary.
其他說明
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