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主要文件

HEL 299

87042207, human lung (embryonic), Fibroblast

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About This Item

分類程式碼代碼:
41106514

产品名称

HEL 299, 87042207

生物源

human lung (embryonic)

增長模式

Adherent

染色體組型

2n = 46, diploid

形態學

Fibroblast

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human embryo lung

細胞系描述

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin. Ethnicity: Black.

應用

HEL 299 has been used to study the antiviral properties of AIC246, a novel anti-human cytomegalovirus compound. It has also been used to study the role of SUMO ligase protein inhibitor of activated STAT1 (PIAS1) in the intrinsic antiviral immune response to herpes simplex virus.

DNA分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818: 11,13
D7S820: 8,11
THO1: 7
TPOX: 8,12
vWA: 16

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Limited lifespan.

其他說明

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