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product name
B92, 85042304
生物源
rat nerve
增長模式
Adherent
染色體組型
Not specified
形態學
Glial/neuronal
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Rat nervous tissue glial
細胞系描述
Ethyl nitrosaurea induced tumour cell line. Neuronal and glial cells are useful for research into the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia. Morphology significantly affected when grown in serum free media.
應用
Neurobiological studies
培養基
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
其他說明
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