OX-80

from Rat x Rat Hybridoma, 13082211

• 分類程式碼代碼: 41106514

属性

• 生物源: Rat x Rat Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: Y3
• 反應: Mouse CD53
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系描述

The hybridoma cell line OX-80 secretes monoclonal IgM antibodies reactive with CD53.

免疫原

RAW 264 cells

應用

The isotype of the monoclonal antibody produced by this cell line is rat IgM.

培養基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

例行更新培養

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.