C6-4-2

8062524, rat brain, Fibroblast

• 分類程式碼代碼: 41106514

属性

• 产品名称: C6-4-2, 08062524
• 生物源: rat brain
• 增長模式: Adherent
• 染色體組型: modal No varied with passage
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Rat glioma x rat glioma hybrid

細胞系描述

Pseudohexadecaploid cell line generated by fusion of the 8-bromodeoxyuridine resistant mutant rat octaploid glioma cell line C6-3-BU-8 and the 6-thioguanine-resistant mutant rat octaploid glioma cell line C6-3-TG-3. C6-4-2 cells provide a suitable model system for studying the regulation of the membrane potential by peptide hormones.

應用

electrophysiology

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C

其他說明

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