Vero (AC-free)

08011101, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• 分類程式碼代碼: 41106514

属性

• 生物源: African green monkey kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 受體: Not specified

说明

一般說明

Vero cells are anchorage-dependent cells derived from the kidney of African green monkey cells. They have applications in molecular and cell biology research. Vero cells are a useful host for the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

細胞系來源

African green monkey kidney cells, serum-free

細胞系描述

Vero cell line (Sigma catalog no. 84113001) adapted to grow in animal component free medium (VP-SFM; Gibco catalog no. 11681-020).

培養基

VP-SFM (Gibco cat no. 11681-020) + 4 mM L-Glutamine (G7513). When freezing cells down use culture medium (50:50 conditioned medium:fresh medium) + 10% DMSO (D2650).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

其他說明

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