T7

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 7021402, mouse lung, Epithelial

• 分類程式碼代碼: 41106514

属性

• 生物源: mouse lung
• 包裝: tube of 5 μg 07021402-DNA-5UG; pkg of vial of cells 07021402-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Mouse Type-II lung epithelial cell

細胞系描述

Fully differentiated Type-II alveolar epithelial cell line derived from transgenic H-2Kb-tsA58 mice capable of being passaged as an immortalised cloned cell line in culture. Cells will proliferate at 33 - 35°C in the presence of gamma-interferon, at 39°C in the absence of gamma-interferon cells will terminally differentiate. Supplied as frozen ampoules only.

培養基

DMEM + 2mM Glutamine + 4% Foetal Bovine Serum (FBS) + 8 additional supplements - please see ′Additional Literature Report′ field below for full details. ECACC can supply an information sheet on the preparation of the supplements on request - contact ECAC

例行更新培養

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;10% CO₂; 33°C. Doubling time 48h

其他說明

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