FTC-236

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6030202, human thyroid (lymph node metastasis), Endothelial

• 分類程式碼代碼: 41106514

属性

• 生物源: human thyroid (lymph node metastasis)
• 包裝: tube of 5 μg 06030202-DNA-5UG; pkg of vial of cells 06030202-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Endothelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Thyroid Cancer Neck Lymph Node Metastasis

細胞系描述

Derived from a neck lymph node metastasis of a follicular thyroid cancer (FTC).This cell line is tumourigenic in Balb/c mice. FTC-133 (Sigma Catalogue number 94060901), FTC-236 (Sigma Catalogue number 06030202) and FTC-238 (Sigma Catalogue number 94060902) were all derived from the same individual, a 42 year old male with follicular thyroid cancer. This has been confirmed by short tandem repeat STR-PCR analysis at ECACC The Y chromosome could not be detected in these cell line by (STR)-PCR analysis. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell lines are identical to the source provided by the depositor based on the STR-PCR analysis.

培養基

DMEM and Ham′s F12 (1:1 mixture) + 10ug/ml Insulin + 0.01U/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

其他說明

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