VP267

5092805, human breast, Epithelial

• 分類程式碼代碼: 41106514

属性

• 产品名称: VP267, 05092805
• 生物源: human breast
• 包裝: tube of 5 μg 05092805-DNA-5UG; pkg of vial of cells 05092805-1VL
• 增長模式: Adherent
• 染色體組型: Mean no. 82, modal no. 62, range 45-148
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Human Breast Cancer

細胞系描述

Derived from a breast cancer biopsy removed from a 48 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade III, tumour cells did not stain positive for oestrogen receptors. The VP267 cell line was derived from the same individual as the cell line VP229. VP229 was derived from the primary tumour and VP267 was derived 14 months later from a local recurrence of the tumour. The individual was recorded to live for 1year after surgery performed to remove the tumour. The patient had received tamoxifen treatment. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,13
D16S539: 11,12
D5S818: 11
D7S820: 10,12
THO1: 7
TPOX: 8
vWA: 17

培養基

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

其他說明

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