Sf21 TitreHigh AC free

5030202, insect ovary, Spherical

• 分類程式碼代碼: 41106514

属性

• product name: Sf21 TitreHigh AC free, 05030202
• 生物源: insect ovary
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Spherical
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

说明

細胞系來源

Spodoptera frugiperda pupal ovarian tissue, serum-free

細胞系描述

This cell line is a derivative of the Sf21 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf21 (Spodoptera frugiperda) insect cell line. The cell line Sf21 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf21 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

應用

Protein expression using the Baculovirus vector system, serum-free

培養基

TiterHigh^™ Sf Insect Medium (Sigma I5408) no supplements.

例行更新培養

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法律資訊

TiterHigh is a trademark of Sigma-Aldrich Co. LLC