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Vero-Hektor

3092503, African green monkey kidney, Fibroblast-like

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About This Item

分類程式碼代碼:
41106514

product name

Vero-Hektor, 03092503, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

生物源

African green monkey kidney

增長模式

Adherent

染色體組型

Not Specified

形態學

Fibroblast-like

受體

Not specified

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細胞系來源

African green monkey kidney cells, serum-free

細胞系描述

Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.

培養基

Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2. Use EDTA only (no trypsin); 5% CO2; 37 °C.

其他說明

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