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product name
Vero-Hektor, 03092503, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
生物源
African green monkey kidney
增長模式
Adherent
染色體組型
Not Specified
形態學
Fibroblast-like
受體
Not specified
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細胞系來源
African green monkey kidney cells, serum-free
細胞系描述
Vero cell line (Sigma catalog no. 84113001) adapted to grow in the chemically defined protein- and peptide-free medium Hektor G (Cell Culture Technologies). This cell line is substrate dependent, i.e., adherent and may be suitable for the replication of viral particles.
培養基
Hektor G medium (Cell Culture Technologies http://www.cellculture.com/) + 4 mM L-Glutamine (G7513). When freezing cells down use Hektor G medium + 4 mM L-Glutamine (G7513) + 10% DMSO (D2650).
例行更新培養
Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 1-3x10,000 cells/cm2. Use EDTA only (no trypsin); 5% CO2; 37 °C.
其他說明
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